Question: Mate_Not_Found after FixMate and filter
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nhs • 0 wrote:
Hi
After applying FixMate and filtering on "isMapped" and "isMatemapped" (and quality and no duplicates) I still have a considerable amount of reads flagged as Mate_Not_Found when running ValidateSam. Mapping to different chromosomes seems not to be the issue judged by Flagstat. How can I get rid of (or salvage) these singletons?
Thanks
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modified 2.6 years ago
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Jennifer Hillman Jackson ♦ 25k
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2.6 years ago by
nhs • 0