Question: tophat sra rna seq
0
gravatar for 1603.neha
2.2 years ago by
1603.neha70
1603.neha70 wrote:

i have sra files from ncbi sra tools i have in my galaxy history. on using tophat i am unable to pair the datasets and execute it. as in the dataset collection it is not showing the paired datasets. i dont have forward or reverse information.how to do it.please help. is it necessary to download files on my computer and then do the tophat analysis. i was following tutorial video from biostar.

rna-seq tophat • 706 views
ADD COMMENTlink modified 2.2 years ago by Jennifer Hillman Jackson25k • written 2.2 years ago by 1603.neha70
0
gravatar for Jennifer Hillman Jackson
2.2 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Double check that the input datatype is a match what the tool is expecting. In this case, fastqsanger is the expected datatype. https://wiki.galaxyproject.org/Support#Tool_doesn.27t_recognize_dataset

Thanks, Jen, Galaxy team

ADD COMMENTlink written 2.2 years ago by Jennifer Hillman Jackson25k

i am using fastqsanger datatype and refernce genome is in gtf

ADD REPLYlink written 2.2 years ago by 1603.neha70
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