Question: Problem with indels not appearing in FreeBayes output
1
gravatar for melora.mackey
2.1 years ago by
melora.mackey10 wrote:

Hello,

I have 6 datasets for 3 individuals (forward and reverse DNA seuqences) that I am trying to identify polymorphic sites in reference to the human genome. I have performed FastQC, Fastq Groomer, Map with Bowtie for Illumina, addorreplacereadgroups, mergeSAMfiles, and performed FreeBayes and performed VCFfilter for -f "QUAL > 40". I am not seeing any insertions or deletions when looking at the FreeBayes data or when filtering for insertions or deletions. Other filters seem to be working such as filtering for snps, mnps, complex, and such. Does anyone know why I cannot see any indels in my output? Thanks in advance!

freebayes • 689 views
ADD COMMENTlink written 2.1 years ago by melora.mackey10
1

HI Do you see indels if you use 'Choose parameter selection> level 1:Simple diploid calling'

If you are using 'Choose parameter selection level>5:Complete list of all options' are you sure that 'Set allelic scope?>Ignore indels alleles' is set to its default no ?

What version of freebayes are you using? Guy

ADD REPLYlink modified 2.1 years ago • written 2.1 years ago by Guy Reeves1.0k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 138 users visited in the last hour