Question: Illumina DNA sequence upload problem
gravatar for lnaren
2.5 years ago by
lnaren10 wrote:

The Illumina DNA sequence for single sample we got was 59 (uncompressed), but I learned that the maximum file you can upload by using FTP should be <50G. Is there any other way I can upload the file to Galaxy?

fastq upload ftp • 779 views
ADD COMMENTlink modified 2.5 years ago by Jennifer Hillman Jackson25k • written 2.5 years ago by lnaren10
gravatar for Jennifer Hillman Jackson
2.5 years ago by
United States
Jennifer Hillman Jackson25k wrote:


Split the fasta file locally, then upload the sub-files. Merge once in a history using the tool Concatenate.

Important considerations:

  • Ensure that fasta data is split logically if you wish to use these sub-files independently (meaning that individual fastq records, four lines each, are each fully intact). But, if the data will only be used in the post-Concatenate-merged format with tools, split fastq entries should not trigger problems as these are disposable intermediate datasets.
  • Large input fastq files may exceed the computing resource at when used with tools. Should this occur, the alternatives outlined here are how to proceed:

Best, Jen, Galaxy team

ADD COMMENTlink modified 10 months ago • written 2.5 years ago by Jennifer Hillman Jackson25k
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