Question: Illumina DNA sequence upload problem
0
gravatar for lnaren
2.1 years ago by
lnaren0
Canada
lnaren0 wrote:

The Illumina DNA sequence for single sample we got was 59 (uncompressed), but I learned that the maximum file you can upload by using FTP should be <50G. Is there any other way I can upload the file to Galaxy?

fastq upload ftp • 669 views
ADD COMMENTlink modified 2.1 years ago by Jennifer Hillman Jackson23k • written 2.1 years ago by lnaren0
4
gravatar for Jennifer Hillman Jackson
2.1 years ago by
United States
Jennifer Hillman Jackson23k wrote:

Hello,

Split the fasta file locally, then upload the sub-files. Merge once in a history using the tool Concatenate.

Important considerations:

  • Ensure that fasta data is split logically if you wish to use these sub-files independently (meaning that individual fastq records, four lines each, are each fully intact). But, if the data will only be used in the post-Concatenate-merged format with tools, split fastq entries should not trigger problems as these are disposable intermediate datasets.
  • Large input fastq files may exceed the computing resource at http://usegalaxy.org when used with tools. Should this occur, the alternatives outlined here are how to proceed: https://galaxyproject.org/support/tool-error/

Best, Jen, Galaxy team

ADD COMMENTlink modified 5 months ago • written 2.1 years ago by Jennifer Hillman Jackson23k
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