I am trying to run the Galaxy Biomina RseQC BAM/SAM mapping quality stats
I have selected my uploaded bam file (generated using Tophat on the main Galaxy instance to map the BodyMap brain tissue paired end fastq files, provided in the shared data).
To make a smaller file for trial analysis, I am using only the reads that map to chr22
(ie. bam>sam> filter for c3=='chr22' and sam>bam)
I have selected 50 for the mapping quality minimum for unique reads (which I believe is the case for Tophat)
I get the following in information> stderror
Fatal error: Exit code 127 (An error occured during execution, see stderr and stdout for more information)
I get blank with stdout
Can anyone suggest what the problem might be?