I've recently received the .fastq files for my rna-seq experiment. According to the sequencing centre, "the samples were run across 2 lanes," and I have received 2 single read .fastq files for each sample.
1) Can somebody explain what it means by "run across 2 lanes"?
2) Does "concatenate datasets tail to head" combine the two files by "merging" the two files on top of each other, or by "connecting" file 1 and file 2? In my case, how would I go about combining the two files before moving onto alignment using Tophat?
Thank you in advance,