Question: BWA - History does not include a dataset of the required format
0
gravatar for Mic
2.9 years ago by
Mic70
Australia
Mic70 wrote:

Hello, I tried to aligned paired end reads with BWA (Map with BWA (version 0.2.3)) on my own Galaxy server. However, I am not able to select any FASTQ files from the history, because I got this error `Select first set of reads: History does not include a dataset of the required format / build`.

This is it the output from FASTQ: 

Filename: SRR512904_1.fastq
File type:Conventional base calls
Encoding: Sanger / Illumina 1.9
Total Sequgences: 23282596
Sequences flagged as poor quality: 0
Sequence length: 76
%GC: 53

Filename: SRR402883_2.fastq
File type: Conventional base calls
Encoding: Sanger / Illumina 1.9
Total Sequences:143366953
Sequences flagged as poor quality: 0
Sequence length: 76
%GC: 53

How is is possible to make the reads visible in BWA?

Thank you in advance.

galaxy • 815 views
ADD COMMENTlink modified 2.9 years ago by Jennifer Hillman Jackson25k • written 2.9 years ago by Mic70
0
gravatar for Jennifer Hillman Jackson
2.9 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Assign the datatype "fastqsanger" to have the datasets available to most tools. Details for how are in the Galaxy wiki: FASTQ_Datatype_QA

Best, Jen, Galaxy team

ADD COMMENTlink written 2.9 years ago by Jennifer Hillman Jackson25k
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