Question: how to male a blast database
0
gravatar for ajmaninisha
2.6 years ago by
ajmaninisha30
Canada
ajmaninisha30 wrote:

Hi,

This is a simple and basic question but am so confused. I have generated my fasta sequence (from assembled transcript files and have used reference annotation file and fasta file for that) and now i want to perform blast2go on the generated fasta sequence . i want to run blast stand alone because blast2go is very slow and i have huge dataset, so i tried using the stand alone.

The first step is to craete a blastdb:

Which file should i use: a generated fasta file or the reference fasta file? Kindly help me , i want to blast my own set of generated sequences.

Any help will be appreciated.

 

Thanks

 

Nisha

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