Question: (Closed) assembled transcripts TO BLAST2GO
gravatar for ajmaninisha
3.5 years ago by
ajmaninisha30 wrote:
Hi , i want to compare the ontologies of genes generated from cuffdiff output and use it for BLAST2GO. Tried several options but for BLAST2GO , a fasta seq is required so i used gffread and then extract genomic DNA for fasta sequence . i have used the merged transcript file generated from cuffmerge . I dont know whether i am following the right track: 1. my aim is to compare the ontologies of differentially expressed genes in 2 samples 2. is there any way to know that which transcripts came from which sample because when i am using the merged transcript file it will give me the fasta seq but will it tell me which seq / transcript from which sample so that i can compare their ontologies in terms of gene expression. Thanks Kindly advise
ADD COMMENTlink modified 3.5 years ago • written 3.5 years ago by ajmaninisha30

Hello ajmaninisha!

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ADD REPLYlink written 3.5 years ago by Jennifer Hillman Jackson25k
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