Dear Sir/Madam,
On the bushman analysis tutorial it states the data set can be filtered on ambiguities, however there no longer appears to be this option in the tools section. How do I get around this problem? Only I have tried other ways and do seem to get the desired results.
Many Thanks,
Emma Sexton
Hello,
The tool "Filter" can be used instead of the legacy tool included in this older published protocol. A video of the procedure is below. Use the same filter expressions in the replacement tool.
http://screencast.g2.bx.psu.edu/galaxy/bushman_analysis/flow.html
Best, Jen, Galaxy team
Hi Jen,
Thanks for your help. Do you know how to account for the absence of the 'what is the meaning of N' as currently I am generating a data set with almost three times as many regions as I should.
Best Wishes
Emma
In response to Galaxy's last question, on the Bushman tutorial the filter expression is: c1 == 'chr22' and c4 != c10 and c10 != 'N'
Following the expression box, there was a box entitled "Meaning of N" in which you would select: everything: A, T, C, G.
This box is not present in just the filter option and this leads to a much larger data set than the one produced in the Bushman example.
Thanks
Rebecca
