Question: normalization gene- rat genome
gravatar for talgolan
3.8 years ago by
talgolan0 wrote:

Hi everyone,

I am looking for a good normalization gene for conducting real time PCR for rat different gene expression.

Does anyone familiar with such?

Until now I used the housekeeping gene GAPDH, but in the current experiment it seems that its expression changes if the culture conditions change.

Thanks for your help,


real time pcr • 697 views
ADD COMMENTlink modified 3.8 years ago by Jennifer Hillman Jackson25k • written 3.8 years ago by talgolan0
gravatar for Jennifer Hillman Jackson
3.8 years ago by
United States
Jennifer Hillman Jackson25k wrote:


It has been a while since I myself have worked with rat data directly, and others may still comment, but I did find this prior Biostars post with some suggested genes to use for normalization, that others are using when performing chip-seq analysis. May point you in the right direction. There could be other posts there of interest - I just did a quick search.

You could also ask your question directly at Biostars - this is the 'Galaxy Biostars' forum and you will not reach as many individuals that work on your same exact research are that can offer general scientific guidance.

Thanks, Jen, Galaxy team

ADD COMMENTlink written 3.8 years ago by Jennifer Hillman Jackson25k
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