I have to analyse the transcriptome data in order to identify differentially expressed genes in the 3 B.rossi strains. There is no reference genome as it has never been sequenced before. I am using CLC Genomics Workbench so I would like to generate a reference. How do I do that? Step by step if possible.
I am new to RNA Seq, so your assistance will be greatly appreciated.
Thanking you in advance.